The Mouse IGF-1 (Insulin-like Growth Factor 1) ELISA Kit - High Sensitivity is an advanced assay meticulously crafted to quantitatively detect IGF-1 levels in various biological samples from mouse models. IGF-1, a vital growth factor, plays a pivotal role in regulating cell proliferation, growth, and tissue repair, making it a key molecule for investigating growth-related mechanisms, aging processes, and disease conditions in mouse studies. This high-sensitivity ELISA kit offers researchers a precise tool to explore IGF-1 dynamics with exceptional accuracy and sensitivity, enabling detailed investigations into its role in modulating cell growth, metabolic functions, and tissue homeostasis with unparalleled precision. The enhanced sensitivity of this kit empowers researchers to delve into the intricate signaling pathways and regulatory functions governed by IGF-1 in mouse models with utmost reliability. The Mouse IGF-1 ELISA Kit - High Sensitivity by Assay Genie provides exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly operation, making it the preferred choice for researchers aiming to study the intricate mechanisms and biological functions of Insulin-like Growth Factor 1 in mouse models.
Product Name:
Mouse IGF-1 ELISA Kit (High Sensitivity)
SKU:
AEES00600
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
