The Mouse IL-1β ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously crafted for the precise and quantitative measurement of Interleukin-1? (IL-1β) levels in various mouse biological samples. IL-1β is a pivotal pro-inflammatory cytokine that orchestrates a myriad of crucial cellular processes, including inflammation, cell proliferation, differentiation, and apoptosis. This high-sensitivity ELISA kit offers unparalleled accuracy and reliability in IL-1β quantification, enabling researchers to unravel the intricate roles of IL-1β in disease mechanisms, autoimmune disorders, and infections. With exceptional sensitivity and specificity, this kit delivers reproducible results, empowering scientists to explore the complexities of IL-1β biology and develop targeted therapeutic interventions. Manufactured under rigorous quality control standards, the Mouse IL-1β ELISA Kit - High Sensitivity showcases robust performance and user-friendly protocols, making it an indispensable tool for cutting-edge research in immunology, inflammation, and cytokine signaling in mouse models.
Product Name:
Mouse IL-1 beta ELISA Kit (High Sensitivity)
SKU:
AEES00590
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.55 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
