Anti-NDUFS1 Antibody (CAB2592)

Antibody Name:	Anti-NDUFS1 Antibody
Antibody SKU:	CAB2592
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-290 of human NDUFS1 (NP_004997.4).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Raji, NCI-H460, PC-3, 293T, HepG2, Mouse kidney, Mouse heart, Mouse intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-290 of human NDUFS1 (NP_004997.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VEIE KAPK VVAA CAMP VMKG WNIL TNSE KSKK AREG VMEF LLAN HPLD CPIC DQGG ECDL QDQS MMFG NDRS RFLE GKRA VEDK NIGP LVKT IMTR CIQC TRCI RFAS EIAG VDDL GTTG RGND MQVG TYIE KMFM SELS GNII DICP VGAL TSKP YAFT ARPW ETRK TESI DVMD AVGS NIVV STRT GEVM RILP RMHE DINE EWIS DKT
Gene ID:	4719
Uniprot:	P28331
Cellular Location:	Mitochondrion inner membrane
Calculated MW:	67kDa/73kDa/75kDa/79kDa/80kDa
Observed MW:	79kDa

Synonyms:

NDUFS1, CI-75Kd, CI-75k, PRO1304

Background:

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NDUFS1: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I 75 kDa subunit family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; EC 1.6.99.3; EC 1.6.5.3; Oxidoreductase; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 2q33-q34 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial respiratory chain complex I Molecular Function:2 iron, 2 sulfur cluster binding; protein binding; NADH dehydrogenase (ubiquinone) activity; electron carrier activity; metal ion binding; 4 iron, 4 sulfur cluster binding Biological Process: ATP metabolic process; cellular metabolic process; apoptotic mitochondrial changes; regulation of mitochondrial membrane potential; mitochondrial electron transport, NADH to ubiquinone; cellular respiration Disease: Mitochondrial Complex I Deficiency
NCBI Summary:	The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:	P28331
NCBI GenInfo Identifier:	92090799
NCBI Gene ID:	4719
NCBI Accession:	P28331.3
UniProt Secondary Accession:	P28331,Q53TR8, Q8N1C4, Q8TCC9, B4DIN9, B4DJA0, B4DPG1 B4DUC1, E7ENF3,
UniProt Related Accession:	P28331
Molecular Weight:	727
NCBI Full Name:	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
NCBI Synonym Full Names:	NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
NCBI Official Symbol:	NDUFS1
NCBI Official Synonym Symbols:	CI-75k; CI-75Kd; PRO1304
NCBI Protein Information:	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; complex I 75kDa subunit; complex I, mitochondrial respiratory chain, 75-kD subunit; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
UniProt Protein Name:	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
UniProt Synonym Protein Names:	Complex I-75kD; CI-75kD
UniProt Gene Name:	NDUFS1
UniProt Entry Name:	NDUS1_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFS1 antibody (CAB2592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using NDUFS1 Rabbit pAb (CAB2592) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using NDUFS1 Rabbit pAb (CAB2592) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using NDUFS1 Rabbit pAb (CAB2592) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-NDUFS1 Antibody (CAB16926)	Human NDUFS1 (NADH-ubiquinone oxidoreductase core subunit S1) ELISA Kit (HUFI07534)
NDUFS1 Antibody (PACO04409)
NDUFS1 Antibody (PACO10794)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)