Description
NHP Cytokine Storm Multiplex Panel 7-plex
Analytes
IL-6, IL-10/CSIF, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and MCP-1/JE/CCL2
Species Reactivity:
Non-Human Primate
Assay Type:
Multiplex
Detection Method:
Flow Cytometry
Assay Time:
2 hours
Product Overview
Cytokine Release Syndrome Multiplex Panel 7-plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IL-6, IL-10, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and CCL2/SYCA2/MCP-1 on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 3 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Product Information
Reactivity: |
Non-Human Primate |
Buffers: |
2x dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent. |
Analytes: |
IL-6, IL-10/CSIF, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and MCP-1/JE/CCL2 |
Lyophilized Standard Mix: |
Non-Human Primate COVID-19 Multiplex Cytokine Immunoassay. One vial containing lyophilized recombinant IL-6, IL-10, IFN-gamma, TNF-alpha, IL-1beta, IL-8/CXCL8, and CCL2/SYCA2/MCP-1 |
Sample Types: |
Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
Standard dose recovery: |
70-130% |
Intra-assay CV: |
< 10% |
Inter-assay CV: |
< 20% |
Cross-reactivity of analytes in the Panel: |
Negligible |
Sample volume: |
15 µL/test |