Anti-Phospho-ATP2A2-Thr484 Antibody (CABP1070)

SKU:
CABP1070
€99 - €319

Description

Technical Manual

Anti-Phospho-ATP2A2-Thr484 Antibody

Product Name:Anti-Phospho-ATP2A2-Thr484 Antibody
Product SKU:CABP1070
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Immunogen:A phospho synthetic peptide corresponding to residues surrounding Thr484 of human ATP2A2.
Sequence:

Email for sequence

Gene ID:488
Swiss Prot:P16615
Synonyms:ATP2B; DAR; DD; SERCA2; ATP2A2/SERCA2; ATP2A2
Calculated MW:109kDa/111kDa/114kDa
Observed MW:114KkDa/140kDa
Reactivity:Human, Mouse, Rat
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:NIH/3T3, C6
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein, Sarcoplasmic reticulum membrane

Product Images

Anti-Phospho-ATP2A2-Thr484 Antibody (CABP1070)

Western blot - Phospho-ATP2A2-Thr484 Rabbit pAb (CABP1070)

Uniprot Information

UniProt Protein Function:SERCA2: an intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. Possesses a Ca(2+)-ATPase activity. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Membrane protein, integral; Transporter; Transporter, ion channel; EC 3.6.3.8; Endoplasmic reticulum; Cell adhesion; Hydrolase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to plasma membrane; membrane; sarcoplasmic reticulum; sarcoplasmic reticulum membrane

Molecular Function:calcium ion binding; calcium-transporting ATPase activity; enzyme binding; protein binding

Biological Process: cell adhesion; elevation of endoplasmic reticulum calcium ion concentration; endoplasmic reticulum calcium ion homeostasis; epidermis development; positive regulation of heart rate

Disease: Acrokeratosis Verruciformis; Darier-white Disease

NCBI Summary:This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]
UniProt Code:P16615
NCBI GenInfo Identifier:114312
NCBI Gene ID:488
NCBI Accession:P16615.1
UniProt Secondary Accession:P16615,P16614, Q86VJ2, A6NDN7, B4DF05,
UniProt Related Accession:P16615
Molecular Weight:109,734 Da
NCBI Full Name:Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
NCBI Synonym Full Names:ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
NCBI Official Symbol:ATP2A2
NCBI Official Synonym Symbols:DD; DAR; ATP2B; SERCA2
NCBI Protein Information:sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Protein Name:Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Synonym Protein Names:Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Protein Family:Sarcoplasmic/endoplasmic reticulum calcium ATPase
UniProt Gene Name:ATP2A2
UniProt Entry Name:AT2A2_HUMAN
View AllClose

Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
View AllClose