The Phospho-LAT (Y171) Polyclonal Antibody (CABP0809) is designed for research involving the phosphorylation of the linker for activation of T cells (LAT) at tyrosine 171. LAT is a key adapter protein involved in T cell receptor signaling pathways, and phosphorylation at Y171 plays a critical role in signal transduction and activation of downstream signaling cascades.The antibody, raised in rabbits, is highly specific for detecting phosphorylated LAT at Y171 in human samples and has been validated for use in Western blot applications.
Its high sensitivity and specificity make it ideal for studying the role of LAT phosphorylation in T cell activation, immune responses, and diseases such as cancer and autoimmune disorders.Understanding the phosphorylation status of LAT at Y171 is essential for unraveling the mechanisms of T cell signaling and immune regulation. This antibody provides researchers with a valuable tool for investigating the role of LAT phosphorylation in various biological processes and potential therapeutic targets for immune-related diseases.
Product Name:
Phospho-LAT-Y171 Rabbit Polyclonal Antibody
SKU:
CABP0809
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y171 of human LAT (NP_001014987.1).
Sequence:
DDYV N
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
LAT1; pp36; IMD52; Phospho-LAT-Y171
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type III membrane protein.
Calculated MW:
28kDa
Observed MW:
38kDa
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
27040
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-LAT-Y171 Rabbit pAb (CABP0809) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 μg/mL) for 2 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 5s.
