Anti-Phospho-MKNK1-T197/202 Antibody (CABP0544)

SKU:
CABP0544
€99 - €319

Description

Technical Manual

Anti-Phospho-MKNK1-T197/202 Antibody

Product Name:Anti-Phospho-MKNK1-T197/202 Antibody
Product SKU:CABP0544
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene.

Immunogen:A phospho specific peptide corresponding to residues surrounding T197/202 of human MKNK1
Sequence:

Email for sequence

Gene ID:8569
Swiss Prot:Q9BUB5
Synonyms:MNK1; MKNK1
Calculated MW:39kDa/47kDa/51kDa
Observed MW:51kDa
Reactivity:Human, Mouse, Rat
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:HeLa, NIH/3T3, PC12
Cellular Location:Cytoplasm, Cytoplasm, Nucleus

Product Images

Anti-Phospho-MKNK1-T197/202 Antibody (CABP0544)

Western blot analysis of extracts of various cell lines, using Phospho-MKNK1-T197/202 antibody (CABP0544) at 1:1000 dilution. HeLa cells were treated by 10% FBS for after serum-starvation overnight or treated by ATP (5mM) at 30'C for 1 hour .NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30'C for 1 hour .PC-12 cells were treated by 20% FBS for 20 minutes after serum-starvation overnight or treated by ATP (5mM) at 30'C for 1 hour Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Uniprot Information

UniProt Protein Function:Mnk1: a CAM kinase of the MAPKKAPK family that can regulate translation. Following activation by MAP kinases, Mnk1 phosphorylates eIF4E at Ser209, increasing its affinity for the 7-methylguanosine-containing mRNA cap. Two MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for its kinase activity. Three alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Translation; Protein kinase, CAMK; CAMK group; MAPKAPK family; MNK subfamily

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function:ATP binding; calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: fibroblast growth factor receptor signaling pathway; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of translational initiation

NCBI Summary:This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:Q9BUB5
NCBI GenInfo Identifier:30316115
NCBI Gene ID:8569
NCBI Accession:Q9BUB5.1
UniProt Secondary Accession:Q9BUB5,O00312, Q5TC06, Q5TC07, Q6V0N6, D3DQ20, D3DQ21
UniProt Related Accession:Q9BUB5
Molecular Weight:39,104 Da
NCBI Full Name:MAP kinase-interacting serine/threonine-protein kinase 1
NCBI Synonym Full Names:MAP kinase interacting serine/threonine kinase 1
NCBI Official Symbol:MKNK1
NCBI Official Synonym Symbols:MNK1
NCBI Protein Information:MAP kinase-interacting serine/threonine-protein kinase 1
UniProt Protein Name:MAP kinase-interacting serine/threonine-protein kinase 1
UniProt Synonym Protein Names:MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1
Protein Family:MAP kinase-interacting serine/threonine-protein kinase
UniProt Gene Name:MKNK1
UniProt Entry Name:MKNK1_HUMAN
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Additional Information

Product type:
Antibody
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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