Anti-Phospho-WWTR1-S89 Antibody (CABP0919)

Antibody Name:	Anti-Phospho-WWTR1-S89 Antibody
Antibody SKU:	CABP0919
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic phosphorylated peptide around S89 of human WWTR1.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	A synthetic phosphorylated peptide around S89 of human WWTR1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	25937
Uniprot:	Q9GZV5
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	44kDa
Observed MW:	Refer to Figures

Synonyms:

WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, TAZ, WWTR1

Background:

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

UniProt Protein Function:	TAZ: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Binds to SLC9A3R2 via the PDZ motif at the plasma membrane. Binds to YWHAZ in vivo and in vitro through the phosphoserine-binding motif RSHSSP. Interacts (via coiled-coil domain) with SMAD2 (via MH1 domain), SMAD3 and SMAD4. Interacts with MED15, PAX8 and NKX2-1. Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
UniProt Protein Details:	Protein type:Oncoprotein; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 3q23-q24 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:protein binding; transcription coactivator activity Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell division; transcription initiation from RNA polymerase II promoter
UniProt Code:	Q9GZV5
NCBI GenInfo Identifier:	67462080
NCBI Gene ID:	25937
NCBI Accession:	Q9GZV5.1
UniProt Secondary Accession:	Q9GZV5,Q8N3P2, Q9Y3W6, D3DNH7,
UniProt Related Accession:	Q9GZV5
Molecular Weight:	44,101 Da
NCBI Full Name:	WW domain-containing transcription regulator protein 1
NCBI Synonym Full Names:	WW domain containing transcription regulator 1
NCBI Official Symbol:	WWTR1
NCBI Official Synonym Symbols:	TAZ
NCBI Protein Information:	WW domain-containing transcription regulator protein 1
UniProt Protein Name:	WW domain-containing transcription regulator protein 1
UniProt Synonym Protein Names:	Transcriptional coactivator with PDZ-binding motif
Protein Family:	WW domain-containing transcription regulator protein
UniProt Gene Name:	WWTR1
UniProt Entry Name:	WWTR1_HUMAN

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