PicoGenie Rat IL-1beta (Interleukin 1 Beta) ELISA Kit
The PicoGenie Rat IL-1beta (Interleukin 1 Beta) ELISA Kit is designed for the quantitative detection of Rat PicoGenie Rat IL-1beta (Interleukin 1 Beta) levels in various biological samples. Interleukin-1 (IL-1) is a principal mediator of innate immunity, produced primarily by activated macrophages and monocytes in response to infection or tissue injury. It drives fever induction, acute-phase protein synthesis, leukocyte recruitment, and activation of adaptive immune responses through NF-κB and MAPK signaling. Dysregulation of IL-1 signaling is central to autoinflammatory syndromes, rheumatoid arthritis, gout, and sepsis-associated organ dysfunction. Assay Genie's PicoGenie Rat IL-1beta (Interleukin 1 Beta) ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 0.2 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's PicoGenie Rat IL-1beta (Interleukin 1 Beta) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
PicoGenie Rat IL-1beta (Interleukin 1 Beta) ELISA Kit
SKU:
AEES02613
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.2 pg/mL
Detection range:
0.31-20 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture Media (n=5)
1:2
Range (%)
86-97
84-95
93-107
Average (%)
92
90
99
1:4
Range (%)
84-95
80-91
93-110
Average (%)
90
86
101
1:8
Range (%)
86-101
80-92
95-111
Average (%)
92
85
101
1:16
Range (%)
87-97
83-93
91-106
Average (%)
92
88
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
84-98
90
Cell Culture Media (n=8)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
2.11
7.27
0.99
1.96
7.35
Standard deviation
0.04
0.12
0.29
0.06
0.16
0.5
C V (%)
4.19
5.63
4.02
6.43
8.32
6.87
Uniport ID:
Q63264
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Rat IL-1β in samples.No significant cross-reactivity or interference between Rat IL-1β and analogues was observed
Storage:
2-8℃
Research Area:
Cancer,Cardiovascular,Metabolism,Immunology
Al-Ghafari,A, B., et al.
Luteolin-loaded Nanoliposomes as a Promising Strategy to Attenuate Hippocampal Aging: Mechanistic Insights into NRF2/HO-1 Activation, Ferroptosis Inhibition, and Mitochondrial Protection
