Rat ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit
The Rat ACE2 (Angiotensin I-Converting Enzyme 2) ELISA Kit is specifically designed for the accurate detection of ACE2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for various research applications.ACE2 is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and cardiovascular function.
It is also the receptor for the SARS-CoV-2 virus, making it a significant target for studying viral infections and developing potential therapies.This ELISA kit is essential for researchers studying ACE2 biology, cardiovascular diseases, viral infections, and other related areas. With its high performance and precision, it is a valuable tool for understanding the role of ACE2 in health and disease.
Product Name:
Rat ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit
SKU:
RTES01078
Target:
Rat ACE2 (Angiotensin I Converting Enzyme 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACE2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACE2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACE2. You can calculate the concentration of Rat ACE2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
94-109
93-105
Average (%)
106
102
99
1:4
Range (%)
92-104
88-100
85-99
Average (%)
98
93
90
1:8
Range (%)
89-104
82-96
82-94
Average (%)
97
87
88
1:16
Range (%)
87-102
80-96
85-96
Average (%)
93
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.91
2.74
8.43
0.99
2.82
8.95
Standard deviation
0.05
0.15
0.34
0.05
0.16
0.42
C V (%)
5.49
5.47
4.03
5.05
5.67
4.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ACE2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ACE2 in samples. No significant cross-reactivity or interference between Rat ACE2 and analogues was observed.
