Rat BMG/ beta2-MG (Beta-2-Microglobulin) ELISA Kit
The Rat BMG Beta2-MG (Beta-2 Microglobulin) ELISA Kit is specifically designed for the accurate and precise measurement of Beta-2 Microglobulin levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for various research applications.Beta-2 Microglobulin is a key protein involved in immune response and kidney function, making it a valuable biomarker for monitoring conditions such as kidney disease, autoimmune disorders, and certain types of cancer.
With the Rat BMG Beta2-MG ELISA Kit, researchers can study the role of Beta-2 Microglobulin in disease progression and potentially uncover new therapeutic targets.
Product Name:
Rat BMG/ beta2-MG (Beta-2-Microglobulin) ELISA Kit
SKU:
RTES00894
Target:
Rat BMG/ beta2-MG (Beta-2-Microglobulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/β2-MG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMG/β2-MG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMG/β2-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMG/β2-MG. You can calculate the concentration of Rat BMG/β2-MG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
100-112
87-98
Average (%)
99
106
92
1:4
Range (%)
101-119
82-93
95-111
Average (%)
108
87
101
1:8
Range (%)
94-110
82-97
96-113
Average (%)
102
89
104
1:16
Range (%)
93-109
85-99
97-108
Average (%)
100
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-107
97
EDTA plasma (n=5)
84-96
90
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.7
9.8
1.1
2.6
9.4
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.4
C V (%)
10
3.7
4.08
9.09
3.85
4.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat BMG/β2-MG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMG/β2-MG in samples. No significant cross-reactivity or interference between Rat BMG/β2-MG and analogues was observed.
