Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
The Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit is a specialized assay designed for the quantitative detection of BMP-2 levels in rat samples. BMP-2 is a crucial protein of the transforming growth factor superfamily, known for its pivotal role in bone formation and regeneration processes. This ELISA kit offers high sensitivity and specificity, allowing accurate measurement of BMP-2 levels in rat biological samples. Detection of BMP-2 is essential for studying bone development, homeostasis, and repair mechanisms, making this kit a valuable tool for research in orthopedics, regenerative medicine, and developmental biology. Manufactured under strict quality control measures, this ELISA kit ensures consistent and reliable results, providing a reliable platform for investigating the physiological functions and therapeutic potentials of BMP-2 in different biological contexts.
Product Name:
Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
SKU:
AEES00286
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.