The Rat DRD1 (Dopamine Receptor D1) CLIA Kit is a highly reliable and sensitive assay designed to accurately detect levels of DRD1 in rat samples. This kit is ideal for researchers studying the role of dopamine receptors in various physiological and pathological processes.The Dopamine Receptor D1 is a key receptor involved in mediating the effects of dopamine, a neurotransmitter that plays a crucial role in regulating various physiological functions such as motor control, reward, and mood.
Dysregulation of DRD1 has been implicated in several neurological and psychiatric disorders, including Parkinson's disease and schizophrenia.With its high sensitivity and specificity, the Rat DRD1 CLIA Kit offers researchers a valuable tool for investigating the role of DRD1 in health and disease. Its ease of use and reproducibility make it suitable for a wide range of research applications in the field of neuroscience and pharmacology.
Product Name:
Rat DRD1 (Dopamine Receptor D1) CLIA Kit
SKU:
RTES00183
Target:
Rat DRD1 (Dopamine Receptor D1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DRD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DRD1. You can calculate the concentration of Rat DRD1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
91-106
90-105
Average (%)
94
97
98
1:4
Range (%)
96-110
103-119
104-120
Average (%)
102
110
110
1:8
Range (%)
98-112
93-108
99-111
Average (%)
106
99
105
1:16
Range (%)
88-100
96-111
84-95
Average (%)
93
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.82
30.9
84.42
10.62
30.48
90.49
Standard deviation
1.17
2.7
6.42
1.18
2.15
6.42
C V (%)
10.81
8.74
7.6
11.11
7.05
7.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DRD1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DRD1 in samples. No significant cross-reactivity or interference between Rat DRD1 and analogues was observed.
