The Rat IL-18 ELISA Kit is engineered to facilitate the precise and quantitative measurement of Interleukin-18 (IL-18) levels in a variety of rat biological specimens. IL-18, a crucial pro-inflammatory cytokine, plays a pivotal role in a broad spectrum of cellular functions, including immune response modulation, cell proliferation, and inflammatory signaling pathways. Its involvement extends to regulating inflammatory cytokine cascades and immune cell activation processes. Crafted by Assay Genie, this IL-18 ELISA Kit ensures remarkable sensitivity and specificity, enabling the generation of accurate and reproducible data. With a focus on stringent quality control benchmarks, this kit assures robust performance and a user-friendly experience, making it an indispensable asset for researchers delving into the intricate roles of IL-18 in diverse physiological contexts.
Product Name:
Rat IL-18 ELISA Kit
SKU:
AEES00458
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
