Rat Insulin Like Growth Factor 1 Receptor (IGF1R) ELISA Kit
The Rat Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA Kit is a powerful tool for precise measurement of IGF1R levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.IGF1R is a key receptor involved in regulating cell growth, differentiation, and survival. It plays a crucial role in various physiological processes, including metabolism, development, and aging.
Dysregulation of IGF1R signaling is associated with numerous diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable target for research and potential therapeutic interventions.With its advanced technology and performance, the Rat IGF1R ELISA Kit is an essential tool for investigating IGF1R-related mechanisms and diseases, paving the way for innovative advancements in biomedical research and drug development.
Product Name:
Rat Insulin Like Growth Factor 1 Receptor (IGF1R) ELISA Kit
Product Code:
RTDL00527
Size:
96 Assays
Target:
IGF1R
Synonyms:
CD221, IGF1-R, IGFIR, JTK13
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
