Rat Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit
The Rat SLC30A3 ELISA Kit is specifically designed for the precise measurement of solute carrier family 30 member 3 (SLC30A3) levels in rat serum, plasma, and cell culture supernatants. This kit boasts a high level of sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.SLC30A3 is a key member of the solute carrier family involved in zinc transport across cell membranes. It plays a critical role in zinc homeostasis and regulation, impacting various physiological processes including immune function, neuronal signaling, and insulin secretion.
Dysregulation of SLC30A3 has been linked to diseases such as diabetes, Alzheimer's, and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat SLC30A3 ELISA Kit offers researchers a reliable tool for investigating the role of SLC30A3 in health and disease, providing valuable insights into zinc metabolism and its impact on various biological pathways.
Product Name:
Rat Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit
Product Code:
RTDL00960
Size:
96 Assays
Target:
SLC30A3
Synonyms:
ZNT3, Zinc Transporter 3
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
