Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit
The Rat TRACP-5B (Tartrate-Resistant Acid Phosphatase 5B) ELISA Kit is specially designed for the precise measurement of TRACP-5B levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent outcomes, making it perfect for a variety of research purposes.TRACP-5B is an enzyme linked to bone metabolism and is essential for osteoclast function.
It is a crucial marker for bone resorption and turnover, playing a key role in bone-related disorders such as osteoporosis and osteoarthritis. The Rat TRACP-5B ELISA Kit is an invaluable tool for investigating bone health and disease progression, providing valuable insights for potential therapeutic interventions.
Product Name:
Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit
SKU:
RTES00780
Target:
Rat TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TRACP-5b. You can calculate the concentration of Rat TRACP-5b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
93-107
86-99
Average (%)
101
100
91
1:4
Range (%)
87-98
85-95
82-97
Average (%)
92
90
88
1:8
Range (%)
91-107
83-94
82-95
Average (%)
99
89
87
1:16
Range (%)
90-101
86-101
83-95
Average (%)
95
93
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
93-109
99
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.07
5.59
19.86
2.26
5.89
20.98
Standard deviation
0.12
0.24
0.68
0.15
0.24
0.72
C V (%)
5.8
4.29
3.42
6.64
4.07
3.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TRACP-5b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TRACP-5b in samples. No significant cross-reactivity or interference between Rat TRACP-5b and analogues was observed.
