Butyrophilin Subfamily 3 Member A1 (BTN3A1/CD277) is a type I transmembrane glycoprotein member of the Ig superfamily. It is expressed on a wide variety of immune cells. Similar to BTN3A2 and BTN3A3, BTN3A1 is composed of an extracellular N-terminal IgV and a membraneproximal IgC domain followed by a transmembrane domain and a cytoplasmic tail. These Ig domains are also found in B7 family costimulatory molecules, suggesting structural and functional similarities between the two protein families. BTN3A1 acts as a critical protein for the activation of V?9V?2 T cells following detection of distressed cells. The anti-tumor responses of V?9V?2 T cells may be enhanced with agonistic anti-BTNA3 antibodies.
Product Name:
Human BTN3A1/CD277 Recombinant Protein (RPES4075)
Product Code:
RPES4075
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Butyrophilin subfamily 3 member A1, CD277, BTN3A1, BTF5
Accession:
O00481
Sequence:
Gln30-Gly254
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human BTN3A1 is produced by our Mammalian expression system and the target gene encoding Gln30-Gly254 is expressed with a Fc tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
50.8 kDa
AP Mol Mass:
55 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
