Haptoglobin(HP) is a secreted protein which is a member of the peptidase S1 family of serine proteases. As a result of hemolysis; hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures; and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin; also known as zonulin; plays a role in intestinal permeability; allowing intercellular tight junction disassembly; and controlling the equilibrium between tolerance and immunity to non-self antigens.
Product Name:
Human Haptoglobin/Zonulin Recombinant Protein (RPES0239)
Product Code:
RPES0239
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Haptoglobin,Zonulin,HP
Accession:
P00738
Sequence:
Val19-Gln160&Ile162-Asn406
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Haptoglobin/Zonulin is produced by our Mammalian expression system and the target gene encoding Val19-Gln160(alpha chain)&Ile162-Asn406(beta chain) is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
15.9&28.3
AP Mol Mass:
120 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; 1mM DTT; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
