Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1; IL-4; IL-10 and IFN-? by suppressing the activation and transduction of PI3K/AKT pathway. In addition; coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance; and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule; PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation; cytokine production; and cytolytic function.
Product Name:
Human PD/PDCD1 Recombinant Protein (RPES0304)
Product Code:
RPES0304
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Programmed cell death protein 1,PDCD1,PD-1,hPD-1,CD279,SLEB2,Hsle1
Accession:
Q15116
Sequence:
Pro21-Gln167
Fusion tag:
C-Fc
Activity:
Immobilized Human PD-1-Fc at 1µg/ml(100 ?l/well) can bind Biotinylated Human PD-L1-Fc(Cat: PKSH033557). The ED50 of PD-1-Fc is 38.696ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human PD-1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a Fc tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
43.6 kDa
AP Mol Mass:
60-70 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
