GTP-Binding Protein Rheb (RHEB) is a member of the small GTPase superfamily and encodes a lipid-anchored; cell membrane protein with five repeats of the RAS-related GTP-binding region. Highest levels of RHEB can be found in the skeletal and cardiac muscle; and it is vital in the regulation of growth and cell cycle progression due to its role in the Insulin/TOR/S6K signaling pathway. RHEB stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling; and it activates the protein kinase activity of mTORC1. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form; farnesylation of the protein is required for this activity.
Product Name:
Human RHEB Recombinant Protein (RPES0127)
Product Code:
RPES0127
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
GTP-Binding Protein Rheb, Ras Homolog Enriched in Brain, RHEB, RHEB2
Accession:
Q15382
Sequence:
Met1-Met184
Fusion tag:
N-GST
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human RHEB is produced by our E.coli expression system and the target gene encoding Met1-Met184 is expressed with a GST tag at the N-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
20.4 kDa
AP Mol Mass:
45 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris; 10mM GSH; pH 8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
