Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Description
Background
| Information | Description |
| Product Name: | SARS-Cov-2 Nucleoprotein / NP Protein (His Tag) |
| Size: | 100µg |
| Background: | RPES0048 |
| Activity: | |
| Synonym: | 2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein |
| Protein Construction: | A DNA sequence encoding the SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag. |
| Sequence: | Met1-Ala422 |
| Fusion tag: | C-6His |
| Accession: | NP_828858.1 |
| Species: | Virus |
| Expressed Host: | Baculovirus-Insect Cells |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity: | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin: | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20? for 3 months. |
| Mol Mass: | 47.5 kDa |
| AP Mol Mass: | 47.1 kDa |
| Formulation: | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
