![Western blot analysis with Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (STMA00235) showing detection of Rat SAP97 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (STMA00235) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/280001/757189/SMC-631_SAP97_Antibody_G28_WB_Rat_brain-membrane-lysate_1__40762.1785409589.png?c=2?imbypass=on "Western blot analysis with Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (STMA00235) showing detection of Rat SAP97 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (STMA00235) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
Description
SAP97 Antibody [G28]
SAP97 Antibody [G28] is a rabbit recombinant monoclonal antibody directed against SAP97, offered by Assay Genie for research applications. Reported applications include WB and ICC-IF. Reported reactivity: Human, Mouse and Rat. Supplied as IgG Kappa, purified by MabSelect™ PrismA.
SAP97 (Synapse-Associated Protein 97), a member of the MAGUK family, is a critical scaffolding protein involved in the trafficking and localization of glutamate receptors, which are essential for synaptic plasticity and cognitive function. Mutations in the DLG1 gene encoding SAP97 have been linked to schizophrenia, with studies showing that these mutations enhance glutamatergic synapse strength in the dentate gyrus and impair episodic memory processing. SAP97 also mediates a non-conventional secretory pathway for NMDA receptors via dendritic Golgi outposts, highlighting its unique role in receptor sorting and synaptic regulation. Furthermore, SAP97 regulates the trafficking of ADAM10, a key α-secretase involved in amyloid precursor protein processing, implicating it in Alzheimer’s disease mechanisms. Our Anti-Rat SAP97 recombinant monoclonal antibody has been demonstrated to detect the target in rodent brain lysates and in cell culture lines. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
| Product Name: | SAP97 Antibody [G28] |
| Product SKU: | STMA00235 |
| Size: | 100 µg |
| Target: | SAP97 |
| Host Species: | Rabbit |
| Antibody Type: | Recombinant Monoclonal |
| Clone ID: | G28 |
| Research Areas: | Neuroscience, Ion Channels, Potassium Channels, Voltage-Gated Potassium Channels, Cell Signaling, Cancer |
| Buffer: | PBS pH 7.4, 0.02% Proclin 300, 50% glycerol |
| Storage & Shipping: | Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label. |
| Format: | Liquid |
| Applications: | WB, ICC-IF |
| Antibody Isotype: | IgG Kappa |
| Purification: | MabSelect™ PrismA |
| Concentration: | 1mg/mL |
| Reactivity: | Human, Mouse, Rat |
| Recommended Dilution: | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Specificity: | Detects N-terminal region of SAP97. Does not cross-react against PSD95, Chapsyn-110, or SAP102 |
| Guarantee: | 12 months from date of dispatch |
| Immunogen: | Fusion protein, AA #1-104 (N-terminus) of rat SAP97. |
| Immunogen Species: | Rat |
| Accession Number: | NP_036920.2 |
| Gene ID: | 25252 |
| Swiss-Prot: | Q62696 |
| Cellular Localization: | Cell membrane, Endoplasmic reticulum membrane, Postsynaptic density, Synapse, Cell junction, Cytoplasm |
| Tissue Specificity: | Widely expressed. Brain, Heart, Muscle, Lung, Liver, Epithelial Cells |
| Alternative Names: | SAP97 Antibody, DLGH1 Antibody, DLGH 1 Antibody, DLG1 Antibody, DLG 1 Antibody, Synapse-associated protein 97 Antibody, Disks large homolog 1 Antibody, SAP-97 Antibody, SAP 97 Antibody |
| References: | 1. E. I., Marshall, J., Aoki, C., de Silva, T., Montgomery, J. M., Garner, C. C., & Green, W. N. (2009). SAP97 and CASK mediate sorting of NMDA receptors through a previously unknown secretory pathway. Nature Neuroscience, 12, 1011–1019. https://doi.org/10.1038/nn.2362 2. Kay, Y., Tsan, L., Davis, E. A., Tian, C., Décarie-Spain, L., Sadybekov, A., Pushkin, A. N., Katritch, V., Kanoski, S. E., & Herring, B. E. (2022). Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats. Nature Communications, 13, 798. https://doi.org/10.1038/s41467-022-28430-5 3. Saraceno, C., Marcello, E., Di Marino, D., Borroni, B., Claeysen, S., Perroy, J., Padovani, A., Tramontano, A., Gardoni, F., & Di Luca, M. (2014). SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation. Cell Death and Disease, 5, e1542. https://doi.org/10.1038/cddis.2014.492 |
| Note: | For research use only. |
![Western blot analysis with Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (STMA00235) showing detection of Rat SAP97 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (STMA00235) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/280001/757189/SMC-631_SAP97_Antibody_G28_WB_Rat_brain-membrane-lysate_1__40762.1785409589.png?c=2 "Western blot analysis with Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (STMA00235) showing detection of Rat SAP97 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (STMA00235) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
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