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    CTLA-4 in Breast Cancer: Insights for Immunotherapy(Post)

    The Role of CTLA-4 in Breast Cancer: Implications for ImmunotherapyBreast cancer (BC) remains one of the most prevalent malignancies affecting women worldwide, with significant morbidity and mortalit ...

    Oct-4 Stem Cells(Post)

    Oct-4 stem cells, also known as Octamer-binding transcription factor 4 stem cells, are a fascinating area of study in the field of stem cell research. These stem cells hold great promise due to t ...

    Anti-CTLA-4 Immunotherapy(Post)

    CD Markers Cluster of Differentiation (CD) markers play an important role in the differentiation of B cells, T cells and NK (natural killer) c ...

    4-1BB: Energizing Immune Cells to Overcome Tumor Resistance(Post)

    Immunotherapy has become a central pillar in the fight against cancer, with a focus on enhancing the body’s natural defenses. Among the molecules at the forefront of immunotherapeutic re ...

    Anti-CTLA-4: Unleashing the Power of T Cells in Combination Immunotherapy(Post)

    Immunotherapy has revolutionized cancer treatment by harnessing the body's immune system to fight malignancies. Among the notable advancements in this field is the development of immune ...

    Ipilimumab and Nivolumab: Advancing Research on CTLA-4 in Cancer Therapy(Post)

    What You Need to Know About IpilimumabWhat is Ipilimumab?Ipilimumab is a monoclonal antibody that targets CTLA-4, a protein involved in downregulating the immune system, to boost the bo ...

    How to Thaw Cells: Best Practices for Cell Culture Success(Post)

    Cell culture techniques are fundamental to biomedical research, providing crucial insights into cellular functions, drug discovery, and disease mechanisms. A critical step in cell culture is the ...

    Antigen Presenting Cells (APCs) and cancer immunotherapy(Post)

    Explore antigen presentation's crucial role in adaptive immunity and its impact on cancer immunotherapy, highlighting the diversity and function of MHC class I molecules. Key Takeawa ...

    VEGF: Inflammation and Tumourigenesis(Post)

    What is VEGF? Vascular Endothelial Growth Factors (VEGFs) are a group of homodimeric polypeptides, and are master regulators of vascular development, maintenance and ...

    Compatibility and Performance Assessment of the GenieColor Mycoplasma Detection Kit Across Diverse Laboratory Conditions(Post)

    MORV0013 functioned effectively in both qPCR machines and water baths, with or without paraffin oil, maintaining consistent sensitivity and specificity. MORV0013 performed reliably betw ...

    Unraveling the Mysteries of Platelet-Activating GPCR Signaling(Post)

    Platelet activation plays a pivotal role in hemostasis, the process that stops bleeding and initiates wound healing. Central to this process is the activation of G protein-coupled receptors (GPCR ...

    Freezing Stimulated T Cells: A Detailed Guide(Post)

    Cryopreservation of stimulated T cells is a common technique in immunology research, allowing for long-term storage while maintaining their functional viability. Stimulated T cells—espec ...

    Transformation Protocol – Sample Transformation Protocols(Post)

    Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bac ...

    BDNF in inflammation and neuropathologies(Post)

    BDNF Overview Brain-derived neurotrophic factor (BDNF) is a central mediator of neuroplasticity, a term used to describe the ability of neuron ...

    Interleukin-8 signalling(Post)

    IL-8 Overview Interleukin-8 (IL-8), also known as CXCL8, was discovered over 30 years ago as a small molecule that could promote chemotaxis and induce the production ...

    Guide to Bacterial Transformation: The Science of Genetic Manipulation(Post)

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    Wnt Signalling Pathway in Immunity(Post)

    What is Wnt Signalling? The Wnt signalling pathway is an evolutionarily conserved pathway that plays critical roles in the regulation of determining cell ...

    Lusvertikimab: Unveiling the Potential in Immuno-Oncology(Post)

    What You Need to Know About LusvertikimabWhat is Lusvertikimab?Lusvertikimab is a monoclonal antibody designed to inhibit the IL-4 receptor, a key player in regulating immune responses, ...

    CD155: Breaking Immune Suppression in Tumor Environments(Post)

    Introduction to CD155 in Cancer ImmunotherapyCD155, also known as Poliovirus Receptor (PVR), is a transmembrane protein that plays a pivotal role in tumor immune evasion. While CD155 is ...

    CD80: Amplifying Immune Activation Through Co-Stimulatory Pathways(Post)

    Introduction to CD80 and Its Role in Immune Activation CD80 is a critical immune checkpoint molecule that plays an essential role in T cell activation through co-stimulatory pathway ...

    CD86: Enhancing T Cell Activation in Immunotherapy(Post)

    Introduction to CD86 and Its Role in Immune Activation CD86 is a crucial co-stimulatory molecule that plays an essential role in T cell activation, driving the immune system’s abili ...

    Targeting Immune Checkpoints as Cancer Therapy(Post)

    In recent years, the field of oncology has witnessed a paradigm shift with the advent of immunotherapy, a treatment modality that harnesses the body's immune system to combat cancer. Among the mo ...

    Utomilumab: Advancing Immuno-Oncology Research(Post)

    Quick Facts About UtomilumabWhat is Utomilumab?Utomilumab is a monoclonal antibody targeting 4-1BB (CD137), a co-stimulatory receptor on T-cells, designed to enhance anti-tumor immunity ...

    Targeting Immune Checkpoints as Cancer Therapy(Post)

    The advent of immune checkpoint targeting marks a significant milestone in the oncological field, offering a beacon of hope for patients battling cancer. This innovative approach leverages the bo ...

    Dupilumab: Transforming Immunotherapy and Skin Disease Management(Post)

    What You Need to Know About DupilumabWhat is Dupilumab?Dupilumab is a fully human monoclonal antibody designed to treat various chronic inflammatory diseases by inhibiting key cytokines ...

    Immune-Checkpoint Inhibition in Cancer Prevention(Post)

    Harnessing Immune-Checkpoint Inhibition for Cancer Prevention Immune-checkpoint inhibition (ICI) has emerged as a pivotal strategy in cancer therapy, particularly in the context of t ...

    Enfortumab Vedotin: Unveiling Its Role in Cancer Treatment and the Rise of Biosimilars(Post)

    Quick Facts About EnfortumabWhat is Enfortumab Vedotin?Enfortumab vedotin is an innovative cancer therapy, primarily used in treating urothelial carcinoma (bladder cancer) that has not ...

    Hemocytometer Cell Counting Procedure(Post)

    Understanding the Functionality of a Hemocytometer Hemocytometers have be ...

    TNFRSF9: Enhancing Immune Cell Activity Against Tumors(Post)

    Introduction to TNFRSF9 and Its Role in Immune ActivationTNFRSF9, also known as 4-1BB or CD137, is a co-stimulatory receptor expressed on the surface of T cells, natural killer (NK) cell ...

    Protease vs Peptidase: Understanding Enzymatic Digestion(Post)

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    3D Organoid Cell Viability Assay: Assessing Environmental Stress on BENOs for Live Delivery(Post)

    3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly ...

    ICOS: Stimulating the Immune System for Superior Cancer Therapy(Post)

    Immunotherapy has transformed the landscape of cancer treatment by leveraging the body’s immune system to target and destroy cancer cells. A key player in this process is Inducible T-cel ...

    The Role of Claudins in Cancer (Post)

    Introduction Claudins are a family of proteins that play a critical role in the formation of ...

    Top 10 Universities in the UK(Post)

    Before I even started my PhD I was already thinking about carrying out PostDoc in a foreign country and had my sights set on going to the US. I had previously carried out a degree in Genetic ...

    TIGIT: A New Frontier in Cancer and Autoimmune Disease Immunotherapy(Post)

    TIGIT (T cell immunoreceptor with Ig and ITIM domains) has emerged as a promising target in cancer immunotherapy and the treatment of autoimmune diseases. As a checkpoint receptor, TIGIT ...

    LILT: A Novel Immune Checkpoint for Cancer Therapy(Post)

    Recent advances in immunotherapy have highlighted the importance of targeting immune checkpoints to enhance the immune system’s ability to combat cancer. A new potential target in this g ...

    10 Steps of Glycolysis: Enzymes, Diagram & ATP Yield(Post)

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    Embryonic Induced Pluripotent Stem Cell Differentiation: Pathways and Lineage-Specific Markers(Post)

    Embryonic induced pluripotent stem cells (iPSCs) hold immense potential in the field of regenerative medicine due to their ability to differentiate into various cell types. Understanding the diff ...

    Unlocking a New Immune Checkpoint: The CD47-Thrombospondin-1 Pathway in Cancer(Post)

    Cancer immunotherapy has revolutionized oncology, offering powerful new ways to treat various malignancies by harnessing the body's own immune system. Central to this success are immune checkpoint in ...

    The Rat Effectively Models Signature Cytokines of T Helper Cells(Post)

    Understanding the immune system's intricacies requires models that accurately reflect its complexity. The rat, as a model organism, plays a crucial role in immunological research, particularly i ...

    TNFR2: Taming Regulatory T Cells to Enhance Anti-Tumor Immunity(Post)

    In recent years, cancer immunotherapy has become a powerful tool for treating various malignancies. One promising approach involves targeting the tumor microenvironment, particularly reg ...

    Targeting VISTA: Unleashing the Power of T Cells in Cancer Therapy(Post)

    V-domain Ig suppressor of T cell activation (VISTA) is an emerging immune checkpoint receptor that plays a key role in suppressing T cell activity within the tumor microenvironment (TME) ...

    ELISA vs Western Blot: Which Technique Should You Choose?(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is f ...

    GITR: Boosting T Cell Activation for Enhanced Cancer Immunotherapy(Post)

    Glucocorticoid-induced tumor necrosis factor receptor (GITR) is an immune checkpoint molecule that plays a crucial role in regulating T cell activation and survival. In recent years, GIT ...

    Sensing Danger: The Biology of TLR-Mediated Inflammation(Post)

    By Eoin Mac Réamoinn Toll-like receptors (TLRs) are renowned for their fundamental roles in innate sensing and initiating inflammatory responses. TLR ...

    CD27 Activation: Strengthening the Immune Army Against Cancer(Post)

    Harnessing the immune system to fight cancer has become a pivotal strategy in modern oncology. A key component in this fight is CD27, a receptor that plays a crucial role in T-cell activ ...

    Physiological Roles for IL-33 | Assay Genie(Post)

    By Charlotte O'Donnell PhD A role for IL-33 in Barrier Function and Epithelial Wound Healing ...

    Toll-like Receptor Signalling in Neurodegenerative Disease(Post)

    Toll-Like Receptors The innate immune response has come under the spotlight in recent years due to its central role in propagating the pathoge ...

    Understanding T Cell Fatigue: Implications for Immune Function and Therapeutic Interventions(Post)

    T cells are central players in adaptive immunity, responsible for recognizing and eliminating infected or aberrant cells. Upon encountering antigens, T cells undergo clonal expansion and diffe ...

    NRF2 Signaling: A Keystone in Inflammation and Disease Management(Post)

    Nuclear Factor Erythroid 2–Related Factor 2 (NRF2) orchestrates a principal defense mechanism against oxidative stress and plays a pivotal role in inflammation and disease pathogenesis. This arti ...

    Early Diagnosis is key: Pick the weed or allow it destroy the garden?(Post)

    By Catríona Dowling, Post-Doc, RCSI I want you to come on a journey with me where we are going to the botanic gardens. You will pick a section of the garden that is yours: yours to treasure ...

    Flow Cytometry Protocol | 10 Hints & Tips(Post)

    Flow Cytometry Protocol The meaning is in the name: Flow = in motion, Cyto = cells, Metry = measure. What is Flow Cytometry? Flow c ...

    CD4+ T Cells: Unveiling the Role of Helper and Regulatory T Cells(Post)

    CD4+ T cells are central to the immune system, functioning as coordinators of immune responses and regulators of immune tolerance. These cells are divided into two primary subtypes: Help ...

    Cellular Respiration: Stages, ATP Production & Pathway Guide(Post)

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    Immunophenotyping: A Comprehensive Analysis of Cellular Immune Profiles(Post)

    Immunophenotyping, a cornerstone of modern immunology, involves identifying and characterizing cell populations based on their surface markers. This technique provides insights into the hetero ...

    Enhancing Cancer Research: Syngeneic Mouse Models and Immune Checkpoint Inhibitors(Post)

    Enhancing Cancer Research: Syngeneic Mouse Models and Immune Checkpoint Inhibitors A quick guide to Syngeneic Mouse Models Syngeneic Mouse Models Cancer research has seen rem ...

    Sandwich ELISA: Step-by-Step Protocol & Troubleshooting(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antib ...

    B7-H4: A New Frontier in Immune Checkpoint Inhibition(Post)

    The recent advances in cancer immunotherapy have brought new opportunities to harness the body’s immune system against tumors. Among these advancements is the targeting of immune checkpo ...

    Mycoplasma Sample Request Form(Page)

    SKU MORV0013 · Mycoplasma Testing GenieColor MycoplasmaDetection Kit Detect Mycoplasma contamination in your cell cultures with a s ...

    인터루킨 8 시그널링(Page)

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    T4 DNA Ligase(Page)

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    IHC Guide(Page)

    Immunohistochemistry Protocol Guide Introduction Immunohistochemistry (IHC) is a technique widely used in histology and pathology ...

    Human Multiplex Inflammation ELISA Panels(Page)

    The ELISA Genie, Human Inflammation multiplex assays utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With multiple sizes of bea ...

    Human Multiplex ELISA Panels(Page)

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    Mouse Multiplex ELISA Panels(Page)

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    Validated GeniePlex Panels(Page)

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    GeniePlex Validated Panels by Cytometer(Page)

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    Validated Multiplex Panels by Flow Cytometer(Page)

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    Distributors - Bahrain(Page)

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    Distributors - United Arab Emirates(Page)

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    CAR-T cell signalling(Page)

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    Regulatory T-cell (Treg) suppression & the IL-2 axis(Page)

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    Distributors - Oman(Page)

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    Adaptive immune system molecules(Page)

    Co-stimulatory and Co-inhibitory Molecules in Adaptive Immunity The adaptive immune system is in part regulated by a vast array of co-stim ...

    Whitepapers(Page)

    White PapersResearchers and companies worldwide trust Assay Genie for high-quality assays and reliable scientific insights. Explore our whitepapers to see how our products perform in re ...

    RIPA Buffer Recipe(Page)

    What is RIPA Buffer? RIPA (Radio Immuno Precipitation Assay) buffer is primarily used when conducting a western blot or immunoprecipatation assay. A RIPA buffer is used in order to l ...

    Co-Immunoprecipitation (co-IP) Protocol(Page)

    Co-immunoprecipitation Protocol A detailed guide of how Co-IP works, a step by step Co-IP protocol and some hints & tips for your Co-IP workflow! Introductio ...

    IHC Buffers & Recipes - Mandarin(Page)

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    B-cell activation and the germinal centre reaction(Page)

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    CD4+ T-helper cell differentiation(Page)

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    T-cell receptor (TCR) signalling(Page)

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    ELISpot Assay Kits(Page)

    ELISpot 400x more sensitive than ELISA! Detection & Visualisation at the Single-Cell Level Explore ELISpot ...

    Interactive Pathway Diagrams(Page)

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    Chemokines(Page)

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    Multiplex Custom Panels(Page)

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    Cytokine neutralisation network(Page)

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    Subcellular Fractionation Protocol - Mitochrondria, Nuclear, Cytosolic(Page)

    What is Subcellular Fractionation? Subcellular fractionation is the process of iIsolating nuclear, cytosolic and mitochondrial fractions of high purity from tissues. Subcellular fracti ...

    T-Cell Co-Stimulation & Agonist Checkpoint Pathway(Page)

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    Non-Human Primate Multiplex ELISA Panels(Page)

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    Multiplex Assays(Page)

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    Cytokine JAK/STAT signalling(Page)

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    PD-1 PD-L1 Pathway Diagram(Page)

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    Subcellular Fractionation Protocol - Mandarin(Page)

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    Rat Multiplex ELISA Panels(Page)

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