The 3-NT (3-Nitrotyrosine) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of 3-nitrotyrosine levels in various biological samples such as serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it an invaluable tool for researchers studying oxidative stress, inflammation, and related diseases.3-Nitrotyrosine is a marker of protein damage caused by reactive nitrogen species, and its levels are elevated in conditions such as inflammatory diseases, neurodegenerative disorders, and cardiovascular diseases. By measuring 3-nitrotyrosine levels, researchers can gain insights into the role of oxidative stress in disease pathogenesis and potentially identify new therapeutic targets.The 3-NT ELISA Kit from Assaygenie offers a convenient and efficient solution for analyzing 3-nitrotyrosine levels in biological samples, allowing researchers to advance their understanding of oxidative stress-related mechanisms and their implications in various disease states.
Product Name:
3-NT (3-Nitrotyrosine) ELISA Kit
SKU:
UNES00025
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
