Description
| Product Name: | 5-Color Prestained Protein Ladder (11-250 kDa) | ||||||||
| SKU: | ABWB0001 | ||||||||
| Number of Bands: | 11 recombinant proteins | ||||||||
| Molecular Weight Range: | 11–250 kDa | ||||||||
| Band Colors: | Blue (majority), Red (80 kDa), Green (25 kDa), Yellow (15 kDa), Purple (250 kDa) | ||||||||
| Usage Recommendation: | 3 μL per lane for 15-well gels, 5 μL per lane for 10-well gels, or 2.5 μL per lane for Western transfer | ||||||||
| Quality Control: | Each batch undergoes strict quality testing. A 5 μL sample of PMX11 resolves 10 visible bands on 4–20% SDS-PAGE (Tris-glycine buffer) and produces distinct reference bands after Western blot transfer to PVDF membranes. | ||||||||
| Storage Buffer Composition: | Each protein band (0.2–0.4 mg/mL) is stored in a stabilizing buffer containing:
|
||||||||
| Storage: |
|
The 5-Color Prestained Protein Ladder (11-250 kDa) is a combination of 11 pre-stained proteins with molecular weights from 11 to 250 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while a red band at 80 kDa, a green band at 25 kDa, a newly designed yellow band at 15 kDa, and a purple band at 250 kDa serve as reference bands. The 5-Color Prestained Protein Ladder (11-250 kDa) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Refer to the 5-Color Prestained Protein Ladder (11-250 kDa) migration pattern under various electrophoresis conditions.
Load 3 μL or 5 μL per lane for optimal visualization on 15-well or 10-well mini-gels, respectively, or 2.5 μL per lane for standard Western transfers.
Note: Molecular weights were determined relative to an unstained protein ladder under each electrophoresis condition. For precise protein sizing, compare with additional calibration data.
Materials Required but not provided
- Vertical Electrophoresis system
- Power supplies
- Vortex or equivalent
- Microcentrifuge
Figure 1. This Assay Genie marker was evaluated using an accelerated stability test by storing samples at 25°C for a designated period.
The conversion is as follows:
- Accelerated 9 days ≈ −20°C for 204 days (0.6 years)
- Accelerated 17 days ≈ −20°C for 385 days (1.1 years)
- Accelerated 34 days ≈ −20°C for 769 days (2.1 years)
- Accelerated 41 days ≈ −20°C for 928 days (2.5 years)
Thermo-Novex 4–20% TG gel was used to run SDS-PAGE in Tris-glycine buffer. 5 μL of protein ladder sample was loaded into each lane.
