Alanine Colorimetric/Fluorometric Assay Kit (BN00876)

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SKU:
BN00876
  • Alanine Colorimetric/Fluorometric Assay Kit (BN00876)
  • Alanine Colorimetric/Fluorometric Assay Kit (BN00876)
€553

Description

ELISA Kit Technical ManualMSDS

Alanine Colorimetric/Fluorometric Assay Kit

Alanine is the 2nd most abundant of the 20 proteinogenic amino acids. Nonessential, being available from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index. Assay Genie's Alanine Assay Kit provides a sensitive detection method of alanine. In the kit, alanine is converted to pyruvate which is specifically detected leading to proportional color (570nm: 0-10 nmol) or fluorescence (Ex/Em 535/587nm: 0-1 nmol) development. Serum concentration: ~24-76 µg/ml (~3-9 nmol/10 µl).

Figure 1: Alanine Colorimetric/Fluorometric Standard Graphs Obtained by following kit protocol.

Key Information Description

Product SKU

BN00876

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The assay can detect serum concentration: ~24-76 µg/ml (~3-9 nmol/10 µl).

Features and Benefits

  • Simple procedure; takes ~ 40 minutes
  • Fast and convenient
  • Kit contains all necessary reagents for accurate measurement of alanine levels

Kit Components

  • Alanine Assay Buffer
  • Alanine probe (in DMSO)
  • Alanine Converting Enzyme
  • Alanine Development Mix
  • Alanine Standard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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