Alpha Synuclein N-terminal Antibody [11D4] is a mouse monoclonal antibody directed against Alpha Synuclein N-terminal, offered by Assay Genie for research applications. Reported applications include WB and IHC. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by Protein G.
Alpha-synuclein is a presynaptic neuronal protein implicated in the pathogenesis of several neurodegenerative diseases, including Parkinson’s disease and dementia with Lewy bodies. Among its structural domains, the N-terminal region plays a pivotal role in membrane binding, conformational dynamics, and aggregation behavior. Post-translational modifications, particularly C-terminal truncations, significantly influence alpha-synuclein’s aggregation propensity and neurotoxicity (1). C-terminally truncated alpha-synuclein species are highly enriched in pathological inclusions such as Lewy bodies and Lewy neurites. These truncated forms exhibit accelerated fibrillization and enhanced prion-like propagation in cellular and animal models of Parkinson’s disease, underscoring their pathogenic relevance (1). Importantly, such truncations can alter the structural conformation of alpha-synuclein, masking epitopes commonly targeted by antibodies, including those recognizing phosphorylated serine 129 (pSer129). This epitope masking has critical implications for research and diagnostics. Antibodies specific to pSer129 may fail to detect C-terminally truncated alpha-synuclein, potentially underestimating the burden of pathogenic species in tissue samples. Therefore, careful selection of antibodies that target the N-terminal region is essential for accurate detection and quantification of disease-relevant alpha-synuclein conformers (3). Understanding the structural and functional roles of the alpha-synuclein N-terminus is vital for elucidating disease mechanisms and developing targeted therapeutic strategies in synucleinopathies. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC
Antibody Isotype:
IgG1
Purification:
Protein G Purified
Concentration:
1mg/ml
Reactivity:
Human, Mouse, Rat
Recommended Dilution:
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects the N-terminal region of alpha synuclein
Guarantee:
12 months from date of dispatch
Immunogen:
Alpha synuclein aa 1-20
Immunogen Species:
Human
Accession Number:
P37840
Gene ID:
6622
Swiss-Prot:
P37840
Cellular Localization:
Cytoplasm; membrane-bound to vesicles in neurons
Tissue Specificity:
CNS and peripheral nerves
Alternative Names:
Alpha Synuclein, α-Synuclein, SNCA, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Synuclein alpha, Synuclein alpha 140, Synuclein, alpha (non A4 component of amyloid precursor), isoform NACP140, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease familial 1, Parkinson disease (autosomal dominant, Lewy body) 4, SYN, SYUA_HUMAN
References:
1. Mahul-Mellier, A.-L., Altay, M. F., Maharjan, N., et al. (2024). Dissecting the differential role of C-terminal truncations in the regulation of aSyn pathology formation and the biogenesis of Lewy bodies. bioRxiv. DOI: 10.1101/2024.11.29.625993 2. Bell, R., Vendruscolo, M. (2021). Modulation of the interactions between α-synuclein and lipid membranes by post-translational modifications. Frontiers in Neurology, 12, 661117. DOI: 10.3389/fneur.2021.661117 3. Iyer, A., Roeters, S. J., Kogan, V., Woutersen, S., Claessens, M. M. A. E., & Subramaniam, V. (2017). C-Terminal Truncated α-Synuclein Fibrils Contain Strongly Twisted β-Sheets. Journal of the American Chemical Society, 139(43), 15392-15400. DOI: 10.1021/jacs.7b07403
