The ACE2 Monoclonal Antibody (CAB4612) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced using hybridoma technology, is highly specific and sensitive for detecting ACE2 protein expression in human samples.The ACE2 receptor plays a crucial role in regulating blood pressure, cardiovascular function, and inflammation by cleaving angiotensin II to angiotensin-(1-7). Its overexpression has been linked to increased susceptibility to SARS-CoV-2, the virus responsible for COVID-19.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACE2 Monoclonal Antibody
SKU:
CAB4612
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC50948
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACEH, ACE2
Positive Sample:
IGROV-1, Mouse kidney, Rat kidney
Cellular Localization:
Cell Membrane, Cytoplasm, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
92kDa
Observed MW:
120-135kDa
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible.
Purification Method
Affinity purification
Gene ID
59272
RRID
AB_2863309
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse kidney using ACE2 Rabbit mAb (CAB4612) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
Western blot analysis of lysates from Rat kidney using ACE2 Rabbit mAb (CAB4612) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Immunohistochemistry analysis of paraffin-embedded Human kidney using ACE2 Rabbit mAb (CAB4612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human testis using ACE2 Rabbit mAb (CAB4612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
