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α-Actinin-4 Rabbit Monoclonal Antibody (CAB3379)

SKU:
CAB3379
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ACTININ-4
FSGS
FSGS1
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:α-Actinin-4 Rabbit Monoclonal Antibody
SKU:CAB3379
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human α-Actinin-4 (O43707).
Sequence:MVDY HAAN QSYQ YGPS SAGN GAGG GGSM GDYM AQED DWDR DLLL DPAW EKQQ RKTF TAWC NSHL RKAG TQIE NIDE DFRD GLKL MLLL EVIS GERL PKPE
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:FSGS; FSGS1; ACTININ-4; α-Actinin-4
Positive Sample:HeLa,293T,Jurkat,Mouse brain,Mouse spleen,Rat lung
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cytoplasm, Nucleus.
Calculated MW:105kDa
Observed MW:100kDa

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Purification Method:Affinity purification
Gene ID:81
Clone Number:ARC1959
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using α-Actinin-4 Rabbit mAb (CAB3379) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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