The Coenzyme NAD Monoclonal Antibody (CAB21047) is a high-quality antibody developed for reliable detection and analysis of target proteins. The coenzyme NAD is involved in oxidation–reduction reactions critical for glycolysis, fatty acid oxidation, the TCA cycle, and complex I of the mitochondrial respiratory chain and also is a key regulator of autophagy. At least two different mechanisms are involved. First, the NAD+-dependent deacetylase SIRT1 activates autophagy by directly deacetylating ATG proteins. Under starvation conditions, the increased NAD+/NADH ratio activates SIRT1, which results in stimulation of mitophagy. Second, the hydrogen of NADH can be transferred to NADP+ to form NADPH via the energy-linked transhydrogenase. In the fed state, when the NAD+/NADH ratio falls, NADPH inhibits autophagy by scavenging of ROS via the glutathione peroxidase-glutathione reductase system and by preventing the production of ROS at complex 1 of the respiratory chain. RRID Gene ID CAS:53-84-9 Swiss Prot Synonym
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Species independent samples.
Product Name:
Coenzyme NAD Monoclonal Antibody
SKU:
CAB21047
Size:
100μL, 20μL
Reactivity:
Species independent
Clone Number:
ARC51050
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to Coenzyme.
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
-
Positive Sample:
-
Cellular Localization:
-
Calculated MW:
-
Observed MW:
Refer to figures
The coenzyme NAD is involved in oxidation–reduction reactions critical for glycolysis, fatty acid oxidation, the TCA cycle, and complex I of the mitochondrial respiratory chain and also is a key regulator of autophagy. At least two different mechanisms are involved. First, the NAD+-dependent deacetylase SIRT1 activates autophagy by directly deacetylating ATG proteins. Under starvation conditions, the increased NAD+/NADH ratio activates SIRT1, which results in stimulation of mitophagy. Second, the hydrogen of NADH can be transferred to NADP+ to form NADPH via the energy-linked transhydrogenase. In the fed state, when the NAD+/NADH ratio falls, NADPH inhibits autophagy by scavenging of ROS via the glutathione peroxidase-glutathione reductase system and by preventing the production of ROS at complex 1 of the respiratory chain. RRID Gene ID CAS:53-84-9 Swiss Prot Synonym
Purification Method:
Affinity purification
Gene ID:
CAS:53-84-9
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
The Coenzyme NAD Rabbit mAb (CAB21047) are tested in Dot Blot against NAD and deoxynucleotide,adenosine. m6A 8 - ATAACTGG-m6A-CCGAATGG m6A 9 - ATAACTGGACCGAATGG
