Description
Anti-Human CD14 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD14 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD14 protein, essential for detecting bacterial lipopolysaccharide (LPS), which plays a pivotal role in the innate immune system. It is ideal for research in immunology, infection biology, and related fields.
With a mouse IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), making it perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline to ensure stability and efficacy. Enhance your research with this reliable and versatile antibody. CD14 is a co-receptor expressed primarily on the surface of monocytes, macrophages, and neutrophils. It is critical for the recognition and clearance of bacterial pathogens, enhancing the immune system's ability to respond to infections by binding to LPS from the outer membrane of Gram-negative bacteria. This interaction facilitates the activation of innate immune responses, underscoring its importance in research focusing on host-pathogen interactions and immune response mechanisms. Elevate your experimental outcomes with the precision and reliability of the Anti-Human CD14 In Vivo Antibody from Assay Genie.
| Product Name: | Anti-Human CD14 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0111 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | UCHM-1 |
| Protein: | CD14 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | P35 |
| Isotype: | Mouse IgG2a |
| Reactivity: | Human |
| Applications: | FC, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FC, WB |
| Recommended Usage: | FC The suggested concentration for this UCHM-1 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this UCHM-1 antibody for use in western blotting is 1-10 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone UCHM-1 recognizes an epitope on human CD14. |
| Antigen Distribution: | CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
| Technical Datasheet: | View |
| Protein: | CD14 |
| Ligand/Receptor: | LPS |
| Research Area: | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
| Mouse IgG2a Isotype Control | |
|---|---|
| Clone | C1.18.4 |
| Isotype | Mouse IgG2a |
| Endotoxin Level | Low Endotoxin |
