Anti-Human CD25 (IL-2Rα) In Vivo Antibody

Product Name:	Anti-Human CD25 (IL-2Rα) In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0352
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	7G7B6
Protein:	CD25
Product Type:	Monoclonal Antibody
Synonyms:	IL-2Rα, CD25, Ly-43, p55, Tac
Isotype:	IgG2a
Reactivity:	Human
Applications:	ELISA, FC, IF Staining, In Vivo, IP, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	ELISA, FC, IF Staining, In Vivo, IP, WB
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone 7G7B6 reacts with human IL-2Rα.
Antigen Distribution:	IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes.
Immunogen:	Recombinant human peripheral blood mononuclear cells (PBMCs)
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rgamma (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency.

Protein:	CD25
Pathogen Testing:	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Shipping:	2-8°C
PubMed:	CD25
NCBI Gene Bank ID:	3559
UniProt:	P01589