The IL15RA Antibody (CAB2983) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization techniques, has been specifically validated for use in Western blot applications and is highly reactive with human samples.IL15RA plays a crucial role in immune regulation, particularly in promoting the activation and survival of immune cells. By targeting IL15RA, researchers can gain valuable insights into the mechanisms underlying immune response modulation and its implications in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IL15RA Antibody
SKU:
CAB2983
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
SHES SHGT PSQT TAKN WELT ASAS HQPP GVYP QGHS DTTV AIST STVL LCGL SAVS LLAC YLKS RQTP PLAS VEME AMEA LPVT WGTS SRDE DLEN CSHH L
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD215, CD215/IL-15R alpha
Positive Sample:
THP-1
Cellular Localization:
Cytoplasmic Vesicle Membrane, Endoplasmic Reticulum Membrane, Golgi Apparatus Membrane, Membrane, Nucleus Membrane, Secreted, Single-Pass Type I Membrane Protein, Single-Pass Type I Membrane Protein, Extracellular Space.
Calculated MW:
28kDa
Observed MW:
60kDa
This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.
Purification Method
Affinity purification
Gene ID
3601
RRID
AB_2764794
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells, using CD215/IL-15R alpha Rabbit pAb (CAB2983) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using CD215/IL-15R alpha Rabbit pAb (CAB2983) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
