Anti-Mouse CD127 (IL-7Rα) In Vivo Antibody

Product Name:	Anti-Mouse CD127 (IL-7Rα) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0343
Size:	1 mg
Clone:	A7R34
Protein:	CD127
Product Type:	Monoclonal Antibody
Synonyms:	IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain
Isotype:	IgG2a k
Reactivity:	Mouse
Applications:	B, CyTOF®, FA, FC, IF Staining, IHC FF, In Vivo, IP, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	<0.01 EU/µg as determined by the LAL method
Purity:	>95% monomer by analytical SEC
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	B, CyTOF®, FA, FC, IF Staining, IHC FF, In Vivo, IP, WB
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone A7R34 recognizes an epitope on mouse CD127.
Antigen Distribution:	IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.
Immunogen:	IL-7Ra-IgG1 fusion protein
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.01 EU/µg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	>95% monomer by analytical SEC
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/gamma chain heterodimer. The common gamma chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.

Protein:	CD127
Function:	T cell and immature B cell proliferation and development
Ligand/Receptor:	IL-7
Research Area:	Immunology
Pathogen Testing:	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Shipping:	2-8°C
PubMed:	CD127
NCBI Gene Bank ID:	16197
UniProt:	CD127