Anti-Mouse CD3ε [500A2] In Vivo Antibody

Product Name:	Anti-Mouse CD3ε [500A2] In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0151
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	500A2
Protein:	CD3-epsilon
Product Type:	Monoclonal Antibody
Synonyms:	T3, CD3
Isotype:	Syrian Hamster IgG2a κ
Reactivity:	Mouse
Immunogen:	Mouse T-cell receptor
Applications:	B, FC, IHC FF
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0150	Anti-Mouse CD3-epsilon [500A2] In Vivo Antibody - Low Endotoxin	Low
IVMB0151	Anti-Mouse CD3ε [500A2] In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, FC, IHC FF
Recommended Usage:	FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Syrian Hamster
Specificity:	Clone 500A2 recognizes an epitope on mouse CD3-epsilon.
Antigen Distribution:	CD3-epsilon is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Immunogen:	Mouse T-cell receptor
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Technical Datasheet:	View
Protein:	CD3-epsilon
Ligand/Receptor:	Peptide antigen/MHC-complex.
Research Area:	Cell Biology, Immunology, Signal Transduction

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Shane Costigan

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