Anti-Mouse CD70 (Clone TAN1-7) In Vivo Antibody

Product Name:	Anti-Mouse CD70 (Clone TAN1-7) In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0373
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	TAN1-7
Protein:	CD70
Product Type:	Monoclonal Antibody
Synonyms:	CD27 Ligand, TNFSF7, Tumor Necrosis Factor Ligand 8A, CD27L, CD70 Antigen, CD27LG, Surface Antigen CD70, CD70 Molecule, Ki-24 Antigen; CD70 Antigen; TNLG8A
Isotype:	IgG2a k
Reactivity:	Mouse
Applications:	B, ELISA, FA, FC, IF, In Vivo
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	B, ELISA, FA, FC, IF, In Vivo
Reactivity:	Mouse

Host Species:	Mouse
Specificity:	Clone TAN1-7 monoclonal antibody recognizes a direct epitope on mouse CD70 and capable of blocking the CD70-CD27 interaction involved in B and T cell activation.
Antigen Distribution:	CD70 is expressed on activated T cells, B cells, and dendritic cells.
Immunogen:	Recombinant mouse CD70 (extracellular domain, aa residues 41-195)
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD70 antibody, TAN1-7, recognizes CD70, a type II membrane protein and member of the TNF family. CD70 is the ligand for the T cell costimulatory receptor CD27 (also known as TNFRSF27)1 and is expressed on activated T cells, B cells, and dendritic cells (DCs)1,2. Ligation of CD27 on T cells with CD70 on DCs provides costimulatory signals and promotes CD4 and CD8 T cell activation3, differentiation4, and survival5,6. CD70 also regulates B cell activation and antibody production7 and enhances natural killer (NK) cell survival and function8. CD70 is constitutively expressed in several hematological and solid tumors and increases the proliferation and survival of tumor cells and regulatory T cells (Tregs), suggesting it may be an effective therapeutic target9-13. Increased levels of CD70 are also associated with autoimmune disorders, including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)14,15.

Protein:	CD70
Research Area:	Costimulatory Molecules, Immunology
Pathogen Testing:	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Shipping:	2-8°C
PubMed:	CD70
NCBI Gene Bank ID:	970
UniProt:	P32970