Description
Anti-Mouse IL-1R (CD121a) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse IL-1R [CD121a] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. This antibody targets the IL-1 receptor (CD121a), a critical component in inflammatory responses, making it ideal for research in immunology and inflammation-related fields. With a rat IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), making it perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. The IL-1 receptor (CD121a) is a key cell surface receptor involved in mediating inflammatory responses. It binds interleukin-1 (IL-1), a pro-inflammatory cytokine, playing a vital role in immune responses by activating downstream signaling pathways that lead to inflammation and immune activation. This receptor is expressed on various immune cells including macrophages, neutrophils, and certain T-cell subsets, and is pivotal in studies focusing on inflammatory diseases and immune regulation.
| Product Name: | Anti-Mouse IL-1R (CD121a) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0006 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | JAMA-147 |
| Protein: | Interleukin-1 R |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD121a |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | Extracellular domain of mouse IL-1 R type 1 |
| Applications: | B, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, IP, WB |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Armenian Hamster Anti-Mouse IL-1 R1 (Clone JAMA-147) recognizes Mouse IL-1 R1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Immunogen: | Extracellular domain of mouse IL-1 R type 1 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
IL-1 binds two receptors, IL-1RI and IL-1RII. The ligands IL-1α and IL-1β exert all of their various biological actions by binding IL-1RI. IL-1RII has a shortened cytoplasmic domain that is unable to transduce signals and therefore acts as a decoy receptor and competitively antagonizes the binding of IL-1α and IL-1β, thereby reducing their biological effects. Binding of the ligands to IL-1RI is enhanced by an accessory protein, IL-1R-AcP. IL-1RI is expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. It is an 80 kD transmembrane glycoprotein.
| Technical Datasheet: | View |
| Protein: | Interleukin-1 R |
| Ligand/Receptor: | IL-1α and IL-1β |
| Research Area: | Immunology, Innate Immunity |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG |
| Endotoxin Level | Low Endotoxin |
