Anti-Mouse IL-4 (Clone 11B11) In Vivo Antibody

Product Name:	Anti-Mouse IL-4 (Clone 11B11) In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0374
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	11B11
Protein:	IL-4
Product Type:	Monoclonal Antibody
Synonyms:	Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, IaIF, HCGF, MCGF2, MFF, TCGF-2
Isotype:	IgG1 k
Reactivity:	Mouse
Applications:	CyTOF®, ELISA Cap, ELISPOT, FA, ICC, IHC, In Vivo, IP, N
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	CyTOF®, ELISA Cap, ELISPOT, FA, ICC, IHC, In Vivo, IP, N
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 11B11 recognizes an epitope on mouse IL-4.
Antigen Distribution:	IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils.
Immunogen:	Purified Recombinant Mouse IL-4
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils1,2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells3,4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes5. In addition, IL-4 is implicated in normal wound healing6,7 and airway inflammation in patients with asthma8.

Protein:	IL-4
Research Area:	Immunology
Pathogen Testing:	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Shipping:	2-8°C
PubMed:	IL-4
NCBI Gene Bank ID:	16189
UniProt:	IL-4