Description
Phospho-MAP3K5-S967 Antibody (CABP1217)
The Phospho-MAP3K5-S967 Antibody (CABP1217) is a high-quality antibody developed for reliable detection and analysis of target proteins. MAP3K5, also known as apoptosis signal-regulating kinase 1 (ASK1), is a key regulator of cellular stress responses and apoptosis pathways. Phosphorylation at serine 967 is known to modulate the activity of MAP3K5, making this antibody a valuable tool for studying the signaling pathways involved in stress-induced apoptosis.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By specifically targeting the phosphorylated form of MAP3K5 at serine 967, researchers can accurately detect and analyze the activation of this important signaling molecule in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | Phospho-MAP3K5-S967 Antibody |
| SKU: | CABP1217 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | ASK1, MEKK5, MAPKKK5, Phospho-MAP3K5-S967 |
| Positive Sample: | Jurkat, Jurkat treated with Hydrogen Peroxide |
| Cellular Localization: | Cytoplasm, Endoplasmic Reticulum. |
| Calculated MW: | 155kDa |
| Observed MW: | 155kDa |
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
| Purification Method | Affinity purification |
| Gene ID | 4217 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Jurkat cells, using Phospho-MAP3K5-S967 Rabbit pAb (CABP1217) at 1:1000 dilution. Jurkat cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour. Jurkat cells were treated with Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s. |
