Ascorbic Acid Assay Kit - Information
Assay Genie's ascorbic acid assay kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H
2O
2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.
Applications
For quantitative determination of ascorbate and evaluation of drug effects on ascorbic acid metabolism.
Ascorbic Acid Assay Kit - Key Features
Use 20 samples. Linear detection range: colorimetric assay 6 to 1000 , fluorimetric assay 1 to 100 ascorbic acid.
Ascorbic Acid Assay Kit -Data Sheet | |
| Kit Includes | Assay Buffer: 10 mL Enzyme Mix: 120 mL Dye Reagent: 120 mL Standard: 400 mL 10 mM ascorbic acid |
| Kit Requires | Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader. |
| Method of Detection | OD570nm, or FL530/585nm |
| Detection Limit | OD, FL: 6, 1 |
| Samples | Serum, plasma, urine, saliva, milk, tissue, and cell culture |
| Species | All |
| Protocol Length | 10 min |
| Size | 100 tests |
| Storage | Store all components at -20°C. |
| Shelf Life | 6 months |
More Details
Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages.
