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Ascorbic Acid Assay Kit (BA0077)

SKU:
BA0077
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine
Research Area:
Oxidative Stress
Antioxidant
Diabetes & Obesity
Food Safety & Analysis
Plant & Environmental Stress
Clinical Chemistry
€919
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Ascorbic Acid Assay Kit - Information

Assay Genie's ascorbic acid assay kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.

Applications

For quantitative determination of ascorbate and evaluation of drug effects on ascorbic acid metabolism.

Ascorbic Acid Assay Kit - Key Features

Use 20 samples. Linear detection range: colorimetric assay 6 to 1000 , fluorimetric assay 1 to 100 ascorbic acid.

Ascorbic Acid Assay Kit -Data Sheet

Kit IncludesAssay Buffer: 10 mL Enzyme Mix: 120 mL Dye Reagent: 120 mL Standard: 400 mL 10 mM ascorbic acid
Kit RequiresPipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader.
Method of DetectionOD570nm, or FL530/585nm
Detection LimitOD, FL: 6, 1
SamplesSerum, plasma, urine, saliva, milk, tissue, and cell culture
SpeciesAll
Protocol Length10 min
Size100 tests
StorageStore all components at -20°C.
Shelf Life6 months

More Details

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages.

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