cAMP PharmaGenie ELISA - Kit Information
Cyclic adenosine monophosphate (cAMP, cyclic AMP, or 3',5'-cyclic adenosine monophosphate) is a second messenger important in many biological processes. cAMP is derived from adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, such as transferring into cells the effects of hormones like glucagon and adrenaline, which cannot pass through the plasma membrane. cAMP functions through three main effectors: PKA, the guanine-nucleotide-exchange factor (GEF) EPAC and cyclic-nucleotide-gated ion channels. The intracellular levels of cAMP are regulated by the balance between the activities of two enzymes adenylyl cyclase (AC) and cyclic nucleotide phosphodiesterase (PDE). Cyclic AMP has been shown to be involved in cell growth, differentiation and general metabolism, and it is important for many biological function, especially in the cardiovascular, nervous and immune systems. The measurement of intracellular Cyclic AMP in tissues and cell cultures may help to provide a clearer understanding of the physiology and pathology of many disease states.
cAMP PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS0012 |
| Size: | 96T |
| Application: | This ELISA kit is designed to only detect Cyclic AMP. |
| Target Species: | Human |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 ng/ml |
| Sensitivity: | 4.4 ng/ml |
| Storage/Stability: | Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
The Assay Genie cAMP ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
| 1. | Prepare all reagents, samples and standards as instructed. |
| 2. | Add 100 ul detection antibody to each well. |
| 3. | Incubate 1.5 h at RT or O/N at 4°C. |
| 4. | Add 100 ul standard or sample to each well. |
| 5. | Incubate 2.5 h at RT. |
| 6. | Add 100 ul prepared streptavidin solution. |
| 7. | Incubate 45 min at RT. |
| 8. | Add 100 ul TMB One-Step Substrate Reagent to each well. |
| 9. | Incubate 30 min at RT. |
| 10. | Add 50 ul Stop Solution to each well. |
| 11. | Read plate at 450 nm immediately. |
