Description
Cholesterol Assay Kit (BA0064) (BA0064)
The Cholesterol Assay Kit (SKU: BA0064) provides a simple, direct and automation-ready procedure for measuring total cholesterol. Cholesterol is a sterol and lipid present in cell membranes and transported in the bloodstream of all animals, playing important roles in forming membranes and hormones and in cell signalling. Elevated levels (hypercholesterolaemia) are associated with cardiovascular diseases such as atherosclerosis, whereas low levels (hypocholesterolaemia) may be linked to depression, cancer and cerebral haemorrhage. This assay uses a single working reagent that combines cholesterol ester hydrolysis, oxidation and colour reaction in one step. The colour intensity at 570 nm, or fluorescence intensity at excitation/emission 530/585 nm, is directly proportional to total cholesterol concentration. The room-temperature protocol requires no 37C heater and is readily automated for high-throughput work.
| Product Name: | Cholesterol Assay Kit (BA0064) |
| SKU: | BA0064 |
| Detection Method: | Colorimetric or Fluorometric |
| Detection Range: | Colorimetric 0.1 to 10 mg/dL; fluorometric 0.02 to 2 mg/dL |
| Sample Type: | Serum, plasma and other biological samples |
| Species Reactivity: | All |
| Assay Time: | 30 minutes |
| Kit Size: | 100 Assays |
| Equipment Required: | Microplate reader |
| Storage: | -20C |
| Shelf Life: | 12 months after receipt |
| Shipping: | Gel Pack |
Quantitative colorimetric or fluorometric determination of total cholesterol. A single working reagent combines cholesterol ester hydrolysis, oxidation and colour reaction in one step, read at 570 nm or fluorometrically at excitation/emission 530/585 nm.
- Sensitive and accurate
- Colorimetric linear detection range 0.1 to 10 mg/dL; fluorometric 0.02 to 2 mg/dL
- Convenient room-temperature assay with no 37C heater required
- High-throughput and readily automated
- Direct assays of cholesterol in serum, plasma and other biological samples
- Pharmacology: effects of drugs o