The COX IV Monoclonal Antibody (CAB11631) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in various applications such as Western blot, immunohistochemistry, and immunofluorescence.Cox IV is essential for mitochondrial function and plays a crucial role in cellular respiration, making it a valuable target for studies in bioenergetics, metabolism, and mitochondrial disorders. By targeting Cox IV, researchers can gain insights into mitochondrial dysfunction, oxidative stress, and metabolic diseases, providing potential avenues for therapeutic intervention and drug development.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
COX IV Monoclonal Antibody
SKU:
CAB11631
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2518
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
COX4, COXIV, COX4-1, COXIV-1, MC4DN16, COX IV-1, COX IV
Positive Sample:
HeLa, MCF7, Mouse brain, Mouse heart, Rat brain , Rat heart
Cellular Localization:
Mitochondrion Inner Membrane.
Calculated MW:
20kDa
Observed MW:
17kDa
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
1327
RRID
AB_2861612
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using COX IV Rabbit mAb (CAB11631) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon using COX IV Rabbit mAb (CAB11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using COX IV Rabbit mAb (CAB11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using COX IV Rabbit mAb (CAB11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
