The CR (Creatinine) ELISA Kit from Assay Genie is specifically designed for the quantitative measurement of creatinine levels in human serum, urine, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results that are essential for various research applications.Creatinine is a waste product produced by muscles and filtered out of the blood by the kidneys. Elevated levels of creatinine in the blood or urine can indicate kidney dysfunction or other health issues. This ELISA kit provides researchers with a valuable tool for studying kidney function, monitoring renal diseases, and evaluating the effects of potential treatments.Whether you are studying renal function, monitoring patients with kidney disease, or investigating novel therapeutic strategies, the CR (Creatinine) ELISA Kit from Assay Genie is a reliable and efficient solution for accurate creatinine detection.
Product Name:
Cr (creatinine) ELISA Kit
SKU:
UNES00038
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.75 μg/mL
Detection range:
1.25-80 μg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
