The Cyclophilin B Monoclonal Antibody (CAB4861) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cyclophilin B Monoclonal Antibody
SKU:
CAB4861
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0290
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OI9, CYPB, SCYLP, CYP-S1, HEL-S-39, Cyclophilin B
Positive Sample:
HeLa, HT-29, Hep G2, Mouse liver, Mouse kidney, Mouse pancreas, Rat lung, Rat liver, Rat spleen
Cellular Localization:
Endoplasmic Reticulum Lumen, Melanosome.
Calculated MW:
24kDa
Observed MW:
19kDa
The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.
Purification Method
Affinity purification
Gene ID
5479
RRID
AB_2863365
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cyclophilin B Rabbit mAb (CAB4861) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
