The DHT (Dihydrotestosterone) ELISA Kit is specifically designed for the precise measurement of Dihydrotestosterone levels in various biological samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.Dihydrotestosterone is a potent androgen hormone that plays a crucial role in the development and maintenance of male characteristics. It is involved in various physiological processes, including the regulation of hair growth, muscle development, and sexual function. Dihydrotestosterone has also been implicated in conditions such as male pattern baldness, prostate cancer, and hirsutism, making it an important biomarker for studying these disorders and exploring potential therapeutic interventions.With its reliable performance and comprehensive applications, the DHT ELISA Kit is a valuable tool for researchers in the fields of endocrinology, oncology, and reproductive medicine. Get accurate and reliable results with this cutting-edge assay kit from Assay Genie.
Product Name:
DHT (Dihydrotestosterone) ELISA Kit
SKU:
UNES00021
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.