The GATB Polyclonal Antibody (PACO59836) is a valuable tool for researchers studying GATB, a protein involved in immune regulation and signaling pathways. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the GATB protein, researchers can effectively detect and analyze its expression in various cell types, making it an essential component in immunology and cancer research studies.
GATB is a key player in modulating immune responses and has been implicated in various diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. Research into the function of GATB is crucial for understanding its role in immune homeostasis and for developing potential therapeutic interventions targeting this protein. The GATB Polyclonal Antibody provides researchers with a reliable tool for investigating GATB's function and its implications in disease pathogenesis.
Western Blot. Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate. All lanes: GATB antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 kDa. Observed band size: 62 kDa.
Immunofluorescence staining of HepG2 cells with PACO59836 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO59836 diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Synonyms:
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial (Glu-AdT subunit B) (EC 6.3.5) (Cytochrome c oxidase assembly factor PET112 homolog), GATB, PET112 PET112L
UniProt Protein Function:
PET112L: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the GatB/GatE family. GatB subfamily.Protein type: Translation; Mitochondrial; Ligase; EC 6.3.5.-; RNA-bindingChromosomal Location of Human Ortholog: 4q31.3Cellular Component: mitochondrionMolecular Function: glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; translation factor activity, nucleic acid bindingBiological Process: mitochondrial translation; translation
