Description
Glutathione Peroxidase 2 Monoclonal Antibody (CAB11171)
The Glutathione Peroxidase 2 Monoclonal Antibody (CAB11171) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, specifically recognizes GPX2 in human samples and is suitable for use in a variety of applications, including Western blot and immunohistochemistry.GPX2 is a key antioxidant enzyme that plays a crucial role in protecting cells from damage caused by reactive oxygen species. Dysregulation of GPX2 has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | Glutathione Peroxidase 2 Monoclonal Antibody |
| SKU: | CAB11171 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Clone Number: | ARC1801 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | QFGH QENC QNEE ILNS LKYV RPGG GYQP TFTL VQKC EVNG QNEH PVFA YLKD KLPY PYDD PFSL MTDP KLII WSPV RRSD VAWN FEKF LIGP EGEP FRRY SRTF P | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | GPRP, GPx-2, GI-GPx, GPRP-2, GPx-GI, GSHPx-2, GSHPX-GI, Glutathione Peroxidase 2 |
| Positive Sample: | HT-29, HepG2 |
| Cellular Localization: | Cytoplasm, Cytosol. |
| Calculated MW: | 22kDa |
| Observed MW: | 23kDa |
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 2877 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates, using Glutathione Peroxidase 2 Rabbit mAb (CAB11171) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
